https://hdl.handle.net/20.500.12809/8899 2026-04-19T12:00:47Z 2026-04-19T12:00:47Z Tamfu, Alfred Ngenge İspirli Doğaç, Yasemin Ceylan, özgür Dediu, Andreea Botezatu Dinica, Rodica Mihaela https://hdl.handle.net/20.500.12809/11064 2023-10-31T11:49:57Z 2023-01-01T00:00:00Z

Antibiofilm and Anti-Quorum Sensing Potential of Safely-Synthesized Hydrated Zirconium Oxide-Coated Alginate Beads against Some Pathogenic Bacteria Tamfu, Alfred Ngenge; İspirli Doğaç, Yasemin; Ceylan, özgür; Dediu, Andreea Botezatu; Dinica, Rodica Mihaela Water is essential to life, but access to uncontaminated water remains a global challenge. Metal oxides possess particular characteristics required for removing heavy metals, inorganic and organic pollutants from wastewater as well as inhibiting microorganisms. Zirconium oxide and alginate which are nontoxic materials were used to synthesize hydrated zirconium oxide-alginate coated materials, ZAB-1 (1.5% alginate) and ZAB-2 (2.0% alginate). FT-IR was used to characterize the functional groups while surface morphology was characterized using SEM. XRD was used to characterize the material structure of the resulting composite. Against Chromobacterium violaceum CV12472, minimal inhibitory concentrations (MICs) were 0.625 mg/mL for ZAB-1 and ZAB-2 while against C. violaceum CV026, the MIC values were 0.625 mg/mL and 1.25 mg/mL for ZAB-1 and ZAB-2, respectively. At MIC and sub-MIC concentrations, the synthesized beads inhibited the production of violacein in C. violaceum CV12472 and C. violaceum CV026, indicating that they can reduce QS-mediated virulence factors in bacteria. Antimicrobial activity was evaluated against Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Candida albicans, and Candida tropicalis, and MIC values ranged from 1.25 mg/mL to 10 mg/mL. Biofilm inhibition percentages were relatively high against S. aureus, E. coli, and C. albicans. It is observed that the increase in the alginate amount from 1.5% to 2.0% improves the antimicrobial, anti-QS, and antibiofilm effects. The alginate makes the zirconium oxide particles biocompatible and easily recoverable from water after treatment. ZAB-1 and ZAB-2 materials can therefore be sustainable materials for water treatment since it can inhibit pathogenic bacteria in water and equally satisfy environmental friendliness. The synthesized particles reduced the chances for antimicrobial resistance since they disrupted QS in bacteria and eliminated biofilms, thereby preventing biofouling of microbial communities in water. Future prospects of this study involve biofiltration, that is, the use of the synthesized composite in the development of a safe and compatible biofilter for water purification.

2023-01-01T00:00:00Z Tel-Çayan, Gülsen Deveci, Ebru Çayan, Fatih https://hdl.handle.net/20.500.12809/11042 2023-10-25T13:10:22Z 2023-01-01T00:00:00Z

Study on Phenolic and Organic Acid Compositions and Antioxidant and Enzyme Inhibition Activities of Agaricomycetes Mushroom Species from Turkey Tel-Çayan, Gülsen; Deveci, Ebru; Çayan, Fatih Mushrooms stand out as one of nature’s best gifts among the natural product sources with their diversity, therapeutic values and increasing popularity. In this study, antioxidant (ABTS•+ scavenging, β-carotene-bleaching, cupric-reducing antioxidant capacity (CUPRAC), DPPH• scavenging, and metal chelating assays), and enzyme (butyrylcholinesterase (BChE) and acetylcholinesterase (AChE), α-amylase and α-glucosidase) inhibition activities of the extracts obtained from Coprinus comatus (O.F. Müll.) Pers., Cerrena unicolor (Bull.) Murrill, Inocutis rheades (Pers.) Fiasson & Niemelä and Leptoporus mollis (Pers.) Quél. mushroom species were investigated. The presence of phenolic and organic acid compounds associated with the bioactive properties of the mushroom species was determined by HPLC-DAD. Fumaric acid was found to be prominent compound in C. comatus (43.90 µg/g dw) and C. unicolor (659.9 µg/g dw), vanillin in L. mollis (19.48 µg/g dw), and p-coumaric acid in I. rheades (21.32 µg/g dw). L. mollis methanol extract, as well as higher antioxidant activity than the standards in CUPRAC and β-carotene-bleaching assays, was noted as superior antioxidant active in all assays (except metal chelating). C. comatus possessed the highest inhibition activity on α-amylase (IC50: 0.23 mg/mL for methanol extract), AChE (IC50: 125.50 µg/mL for hexane extract), and BChE (IC50: 61.03 µg/mL for methanol extract). Also, C. comatus methanol (IC50: 0.09 mg/mL) and L. mollis hexane (IC50: 0.11 mg/ mL) extracts were better α-glucosidase inhibition active than the acarbose (IC50: 0.37 mg/mL). Our study ascertained that the studied mushroom species are particularly sources of biochemically active compounds with therapeutic potential.

2023-01-01T00:00:00Z Tel-Çayan, Gülsen https://hdl.handle.net/20.500.12809/11027 2023-10-17T12:53:09Z 2023-01-01T00:00:00Z

Bioactive natural small molecules from mushrooms as potential source of anticancer agents Tel-Çayan, Gülsen Recently, drugs obtained from natural products have attracted great interest as potential therapeutic agents in cancer treatment due to their lesser side effects and low toxicity. Mushrooms are one of the natural sources containing various bioactive compounds with antioxidant, anticancer, antidiabetic, antimicrobial, hepatoprotective, antitumor, antiviral, and immunomodulatory properties. This chapter reviews the anticancer metabolites of low-molecular weight from mushroom species. Low-molecular-weight metabolites have the ability to go into the cell and affect or interfere with the biological processes therein. Research both in vivo and in vitro has reported that such compounds derived from natural sources are efficient in inhibiting cancer growth and metastasis. Low molecular weight cytotoxic molecules such as pulveraven A, roridin E, 12′-episatratoxin H, trichoverritone, astrakurkurone, inotodiol, GL22, lepiotaprocerins H, G, L, vulpinic acid, grifolin, craterellyne I, phellibarin D, trichodermol, 8-deoxytrichothecin, trichothecin, trichothecinol B, trichothecinol A, atractylenolide I, terreumol A, flammulinolides A, B, C, F, jiangxienone, aurisin A, ergosta‐7,22‐diene‐2β,3α,9α‐triol, hypoxylonols D, E, and neogrifolin have been purified from various mushroom species. In this chapter, the compounds (1–257) with anticancer activity isolated from 61 different mushroom species in the last 2 decades were evaluated. Further, triterpenoids, steroids, steroidal alcohols, phenolic compounds, sesquiterpenes, meroterpenoids, p-terphenyls, ergostanes, alkaloids, fatty acids, cerebrosides, furanones, disulfide compounds, butanolides, aromatic compounds, organic acids, benzo[j]fluoranthene derivatives and cyclopeptides indicating cytotoxic activity toward various cancer cell lines from mushrooms were reported here.

2023-01-01T00:00:00Z Şahin, Muradiye Atasoy, Muhammet Arslan, Yasin Yıldız, Dilek https://hdl.handle.net/20.500.12809/11009 2023-10-05T11:47:40Z 2023-01-01T00:00:00Z

Removal of Ni(II), Cu(II), Pb(II), and Cd(II) from Aqueous Phases by Silver Nanoparticles and Magnetic Nanoparticles/Nanocomposites Şahin, Muradiye; Atasoy, Muhammet; Arslan, Yasin; Yıldız, Dilek he intake of heavy metals into the body, even at very low concentrations, may cause a decrease in central nervous system functions; deterioration of blood composition; and liver, kidney, and lung damage. Therefore, heavy metal ions must be removed from water. In this study, silver, magnetic iron/copper, and iron oxide nanoparticles were synthesized using Lathyrus brachypterus extract and then Fe/Cu-AT, Fe3O4-AT, Fe/Cu-CS, and Fe3O4-CS magnetic nanocomposite beads were synthesized using alginate and chitosan. The removal of Cd(II), Pb(II), Ni(II), and Cu(II) ions from aqueous phases using synthesized nanoadsorbents was investigated by single and competitive (double and quaternary) adsorption techniques. The kinetic usability of the magnetic iron oxide chitosan (Fe3O4-CS) nanocomposite beads with the highest removal efficiency was evaluated. Based on experimental results, the order of removal was found to be 98.39, 75.52, 51.54, and 45.34%, and it was listed as Pb(II) > Cu(II) > Cd(II) > Ni(II), respectively. The Dubinin-Radushkevich, Freundlich, Langmuir, and Temkin isotherm models were used, and experimental results revealed that the experimental data fit the Langmuir model better. The maximum adsorption capacities (qm) obtained from the Langmuir isotherm model of Fe3O4-CS were found to be 8.71, 23.75, 18.57, and 12.38 mg/g for Ni(II), Pb(II), Cu(II), and Cd(II) ions, respectively. When the kinetic data were applied to the Lagergren, Ho-McKay, and Elovich models, it was observed that the adsorption kinetics mostly conformed to the Ho-McKay second-order rate equation. The binary and quaternary competitive adsorption data showed that Fe3O4-CS were selective toward Cu(II) and Pb(II). The reusability of the Fe3O4-CS nanoadsorbent was performed as three cycles with the same concentration. The adsorption capacities were found to be 95.81, 70.65, 50.50, and 42.75%, in turn for Pb(II), Cu(II), Cd(II), and Ni(II) ions after three cycles, which revealed that the Fe3O4-CS nanoadsorbent can be used after three cycles without losing its efficiency.

2023-01-01T00:00:00Z