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dc.contributor.authorKaya, Yeşim
dc.contributor.authorYıldız, Ayeşgül
dc.contributor.authorYenilmez Tunoğlu, Ezgi Nurdan
dc.contributor.authorKorulu, Şirin
dc.date.accessioned2023-08-09T12:11:24Z
dc.date.available2023-08-09T12:11:24Z
dc.date.issued2023en_US
dc.identifier.citationKaya Y, Korulu S, Tunoglu ENY, Yildiz A. A potential posttranscriptional regulator for p60-katanin: miR-124-3p. Cytoskeleton (Hoboken). 2023 Jul 13. doi: 10.1002/cm.21769. Epub ahead of print. PMID: 37439368.en_US
dc.identifier.issn19493584
dc.identifier.otherPubMed ID : 37439368
dc.identifier.urihttps://hdl.handle.net/20.500.12809/10872
dc.description.abstractKatanin is a microtubule severing protein belonging to the ATPase family and consists of two subunits; p60-katanin synthesized by the KATNA1 gene and p80-katanin synthesized by the KATNB1 gene. Microtubule severing is one of the mechanisms that allow the reorganization of microtubules depending on cellular needs. While this reorganization of microtubules is associated with mitosis in dividing cells, it primarily takes part in the formation of structures such as axons and dendrites in nondividing mature neurons. Therefore, it is extremely important in neuronal branching. p60 and p80 katanin subunits coexist in the cell. While p60-katanin is responsible for cutting microtubules with its ATPase function, p80-katanin is responsible for the regulation of p60-katanin and its localization in the centrosome. Although katanin has vital functions in the cell, there are no known posttranscriptional regulators of it. MicroRNAs (miRNAs) are a group of small noncoding ribonucleotides that have been found to have important roles in regulating gene expression posttranscriptionally. Despite being important in gene regulation, so far no microRNA has been experimentally associated with katanin regulation. In this study, the effects of miR-124-3p, which we detected as a result of bioinformatics analysis to have the potential to bind to the p60 katanin mRNA, were investigated. For this aim, in this study, SH-SY5Y neuroblastoma cells were transfected with pre-miR-124-3p mimics and pre-mir miRNA precursor as a negative control, and the effect of this transfection on p60-katanin expression was measured at both RNA and protein levels by quantitative real-time PCR (qRT-PCR) and western blotting, respectively. The results of this study showed for the first time that miR-124-3p, which was predicted to bind p60-katanin mRNA by bioinformatic analysis, may regulate the expression of the KATNA1 gene. The data obtained within the scope of this study will make important contributions in order to better understand the regulation of the expression of p60-katanin which as well will have an incontrovertible impact on the understanding of the importance of cytoskeletal reorganization in both mitotic and postmitotic cells.en_US
dc.item-language.isoengen_US
dc.publisherJohn Wiley and Sons Incen_US
dc.relation.isversionof10.1002/cm.21769.en_US
dc.item-rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGene regulationen_US
dc.subjectMicrotubule severingen_US
dc.subjectmiR-124-3pen_US
dc.subjectp60-kataninen_US
dc.titleA potential posttranscriptional regulator for p60-katanin: miR-124-3pen_US
dc.item-typearticleen_US
dc.contributor.departmentMÜ, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.contributor.authorID0000-0002-3601-6931en_US
dc.contributor.authorID0000-0001-6356-7459en_US
dc.contributor.institutionauthorKaya, Yeşim
dc.contributor.institutionauthorYıldız, Ayeşgül
dc.relation.journalCytoskeletonen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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