COMPARISON OF ANTIBIOTIC SENSITIVITY RATIO OF ESHERICHIA COLI AND KLEBSIELLA PNEUMONIAE STRAINS ISOLATED FROM VARIOUS CLINICAL SPECIMENS IN INTENSIVE CARE UNIT

Abstract

A retrospective analysis of the widely used antibiotics all susceptibility testing results from Esherichia coli and Klebsiella pneumoniae cultured from clinical specimens Mugla Sitki Koeman University Educatioan and Research hospital (January to December in 2015) was performed. The new BD PHOENIX automated microbiology system (Becton Dickinson Diagnostic Systems, Sparks, Md.) is designed for automated rapid antimicrobial susceptibility testing and identification of clinically relevant bacteria. Minimum Inhibitory Concentration (MIC) results previously obtained in recent clinical isolates with well-defined in isolates with well-characterized resistance mechanisms with the microdilution method were re-interpreted for the susceptible, intermediate and resistant categories using the 2012 EU-CAST breakpoints. Clinical samples are most commonly isolated from tracheal aspirates, wound site, blood, mucus, abcess, catheter, urine samples, throat and nose cultures. E.coli isolates were highly resistant to Ciprofloxacin (CIP), Cefepime (FEP), and Aztreonam (ATM) 47%, %47 and 40% respectively. Unlike K.pneumoniae isolates were highly resistant to Ampicillin/Sulbactam (SAM), ATM and FEP 58%, 53% and 50% respectively. Both of the bacteria showed the highest sensitivity rate to Amikacin (AN) 98% and 90% E.coli and K.pneumoniae respectively. Out of the 60 E.coli strains 34 (57%) isolates showed Multiple Antibiotic Resistance (MAR) two to ten antibiotics. Out of the 60 K.pneumoniae strains 38 (63%) isolates showed MAR two to ten antibiotics. Considering the antibiogram, AN and Meropenem (MEM) should be preferred drugs for K. pneumoniae and E.coli infection isolated from clinical samples.