Basit öğe kaydını göster

dc.contributor.authorAnık, Ülkü
dc.contributor.authorCubukcu, Meliha
dc.contributor.authorCevik, Serdar
dc.contributor.authorTimur, Suna
dc.date.accessioned2020-11-20T16:34:00Z
dc.date.available2020-11-20T16:34:00Z
dc.date.issued2010
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.urihttps://doi.org/10.1002/elan.200900447
dc.identifier.urihttps://hdl.handle.net/20.500.12809/4548
dc.descriptionanik, ulku/0000-0002-3607-7208en_US
dc.descriptionWOS: 000280172900017en_US
dc.description.abstractA biosensor for monitoring alkaline phosphatase (ALP) enzyme activity was developed by using bismuth film electrode (BiFE) as transducer. The aim was to test the possible applications of BiFE as transducer in this biosensing platform. ALP was immobilized onto the BiFE electrode by means of gelatin which was then crosslinked by glutaraldehyde. The measurement was based on monitoring of p-nitrophenol which was released after enzymatic reaction of p-nitrophenyl phosphate (pNPP) substrate. The effect of enzyme activity (in Unit) was examined for 100 mu M of pNPP substrate and a good linearity was observed between 1.28 and 4.26 U activities with the equation of y = 0.214x - 0.201 and R-2 = 0.998 where x and y show enzyme activity in unit and current response in mu A respectively. Analytical characteristics were also examined after the optimization of experimental parameters.en_US
dc.item-language.isoengen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.item-rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBismuth Film Electrodeen_US
dc.subjectAlkaline Phosphatase (ALP)en_US
dc.subjectBiosensorsen_US
dc.subjectBismuthen_US
dc.titleUsage of Bismuth Film Electrode as Biosensor Transducer for Alkaline Phosphatase Assayen_US
dc.item-typearticleen_US
dc.contributor.departmenten_US
dc.contributor.departmentTemp[Anik, Uelkue; Cubukcu, Meliha; Cevik, Serdar] Mugla Univ, Fac Arts & Sci, Dept Chem, TR-48000 Kotekli, Mugla, Turkey -- [Cubukcu, Meliha] Ege Univ, Fac Sci, Dept Chem, TR-35100 Izmir, Turkey -- [Timur, Suna] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkeyen_US
dc.identifier.doi10.1002/elan.200900447
dc.identifier.volume22en_US
dc.identifier.issue13en_US
dc.identifier.startpage1519en_US
dc.identifier.endpage1523en_US
dc.relation.journalElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


Bu öğenin dosyaları:

DosyalarBoyutBiçimGöster

Bu öğe ile ilişkili dosya yok.

Bu öğe aşağıdaki koleksiyon(lar)da görünmektedir.

Basit öğe kaydını göster