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dc.contributor.authorDemircan, Turan
dc.contributor.authorSibai, Mustafa
dc.contributor.authorAvşaroğlu, Mahmut Erhan
dc.contributor.authorAltuntaş, Ebru
dc.contributor.authorOvezmyradov, Guvanch
dc.date.accessioned2020-11-20T16:50:26Z
dc.date.available2020-11-20T16:50:26Z
dc.date.issued2020
dc.identifier.issn0378-1119
dc.identifier.urihttps://doi.org/10.1016/j.gene.2020.145258
dc.identifier.urihttps://hdl.handle.net/20.500.12809/6251
dc.description.abstractMicroRNAs (miRNAs) are endogenously coded small RNAs, implicated in post-transcriptional gene regulation by targeting messenger RNAs (mRNAs). Circulating miRNAs are cell-free molecules, found in body fluids, such as blood and saliva, and emerged recently as potential diagnostic biomarkers. Functions of circulating miRNAs and their roles in target tissues have been extensively investigated in mammals, and the reports on circulating miRNAs in non-mammalian clades are largely missing. Salamanders display remarkable regenerative potential, and the Mexican axolotl (Ambystoma mexicanum), a critically endangered aquatic salamander, has emerged as a powerful model organism in regeneration and developmental studies. This study aimed to explore the circulating miRNA signature in axolotl blood plasma. Small RNA sequencing on plasma samples revealed 16 differentially expressed (DE) circulating miRNAs between neotenic and metamorphic stages out of identified 164 conserved miRNAs. Bioinformatics predictions provided functional annotation of detected miRNAs for both stages and enrichment of DE miRNAs in cancer-related and developmental pathways was notable. Comparison with previous reports on axolotl miRNAs unraveled common and unique members of the axolotl circulating miRNome. Overall, this work provides novel insights into non-mammalian aspects of circulating miRNA biology and expands the multi-omics toolkit for this versatile model organism. © 2020 Elsevier B.V.en_US
dc.description.sponsorship215S526 Türkiye Bilimsel ve Teknolojik Araştirma Kurumuen_US
dc.description.sponsorshipThis study was supported financially by the Scientific and Technological Research Council of Turkey (TÜBİTAK; project number: 215S526) for which we are thankful.en_US
dc.item-language.isoengen_US
dc.publisherElsevier B.V.en_US
dc.item-rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAxolotlen_US
dc.subjectCirculating miRNAen_US
dc.subjectFunctional annotationen_US
dc.subjectMetamorphosisen_US
dc.subjectmiRNAen_US
dc.subjectSmall RNA sequencingen_US
dc.titleThe first report on circulating microRNAs at Pre- and Post-metamorphic stages of axolotlsen_US
dc.item-typearticleen_US
dc.contributor.departmentMÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.contributor.institutionauthorDemircan, Turan
dc.contributor.institutionauthorAltuntaş, Ebru
dc.identifier.doi10.1016/j.gene.2020.145258
dc.relation.journalGeneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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