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dc.contributor.authorSouza, F.V.D.
dc.contributor.authorde Souza, E.H.
dc.contributor.authorKaya, E.
dc.contributor.authorde Jesus Vieira, L.
dc.contributor.authorda Silva, R.L.
dc.date.accessioned2020-11-20T17:17:25Z
dc.date.available2020-11-20T17:17:25Z
dc.date.issued2018
dc.identifier.issn1064-3745
dc.identifier.urihttps://doi.org/10.1007/978-1-4939-8594-4_18
dc.identifier.urihttps://hdl.handle.net/20.500.12809/6421
dc.descriptionPubMed ID: 29981128en_US
dc.description.abstractCryopreservation is a technique that allows the conservation of many species for long periods. Among the protocols used for cryopreservation, droplet vitrification has shown efficient results in preserving shoot tips of various wild and cultivated pineapple genotypes. The method consists of extraction of shoot tips from plants grown in vitro, dehydration for a period of 48 h in a preculture medium supplemented with a high concentration of sucrose, treatment in a plant vitrification solution (PVS2), and immersion in liquid nitrogen. The method described in this chapter has produced survival and regeneration indices of around 70%, depending on the genotype and physiological conditions of the initial explants. The objective of this chapter is to describe in detail a droplet vitrification protocol for shoot tips that is easy to perform for cryopreservation of pineapple germplasm. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.en_US
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior Conselho Nacional de Desenvolvimento Científico e Tecnológico Empresa Brasileira de Pesquisa Agropecuária Fundação de Amparo à Pesquisa do Estado da Bahiaen_US
dc.description.sponsorshipThe authors acknowledge Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)/ EMBRAPA program, and Embrapa Mandioca e Fruticultura for financial support and Helder Lima Carvalho, technician of the Laboratório de Cultura de Tecido de Plantas, for his valuable collaboration in preparing this chapter.en_US
dc.item-language.isoengen_US
dc.publisherHumana Press Inc.en_US
dc.item-rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnanas comosusen_US
dc.subjectConservationen_US
dc.subjectPlant vitrification solutionen_US
dc.titleCryopreservation of Pineapple Shoot Tips by the Droplet Vitrification Techniqueen_US
dc.item-typebookParten_US
dc.contributor.departmenten_US
dc.contributor.departmentTempSouza, F.V.D., Embrapa Cassava and Fruits, Cruz das Almas, Bahia, Brazil, Federal University of Recôncavo da Bahia (UFRB), Cruz das Almas, Bahia, Brazil; de Souza, E.H., Federal University of Recôncavo da Bahia (UFRB), Cruz das Almas, Bahia, Brazil, Scholarship of Coordination for the Improvement of Higher Education Personnel (CAPES) at CAPES-EMBRAPA Program in Embrapa Cassava and Fruits (CNPMF), Cruz das Almas, Bahia, Brazil; Kaya, E., Department of Molecular Biology and Genetics, Faculty of Science Mugla Sitki Kocman University Koteki, Mugla, Turkey; de Jesus Vieira, L., Scholarship of Coordination for the Improvement of Higher Education Personnel (CAPES) at CAPES-EMBRAPA Program in Embrapa Cassava and Fruits (CNPMF), Cruz das Almas, Bahia, Brazil; da Silva, R.L., State University of Feira de Santana (UEFS), Feira de Santana, Bahia, Brazilen_US
dc.identifier.doi10.1007/978-1-4939-8594-4_18
dc.identifier.volume1815en_US
dc.identifier.startpage269en_US
dc.identifier.endpage277en_US
dc.relation.journalMethods in Molecular Biologyen_US
dc.relation.publicationcategoryKitap Bölümü - Uluslararasıen_US


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