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dc.contributor.authorAlper, Mehlika
dc.contributor.authorGüneş, Hatice
dc.date.accessioned2020-11-20T14:41:21Z
dc.date.available2020-11-20T14:41:21Z
dc.date.issued2019
dc.identifier.issn1304-530X
dc.identifier.urihttps://doi.org/10.4274/tjps.galenos.2018.27146
dc.identifier.urihttps://app.trdizin.gov.tr//makale/TXpVME1qQTNOdz09
dc.identifier.urihttps://hdl.handle.net/20.500.12809/913
dc.descriptionWOS: 000474889900004en_US
dc.descriptionPubMed ID: 32454725en_US
dc.description.abstractObjectives: Natural products originating from plants have been used for many years in the treatment of various diseases, including cancer. Centaurea solstitialis subsp. solstitialis is used in Turkish folk medicine. This study was the first to determine the in vitro biological effects of ethanolic extract from the flowering parts of C. solstitialis L. subsp. solstitialis collected from Mugla Province. Materials and Methods: The cytotoxic effect was evaluated against Daudi, A549, and HeLa cancer cells and one normal BEAS-2B cell line using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- dipenyltetrazolium bromide) assay. Flow cytometric analysis and the caspase-3 activity assay were performed to detect apoptotic cell death. Angiogenic factor [vascular endothelial growth factor (VEGF)] secretion and the release of interleukin (IL)-1 alpha, IL-6, and tumor necrosis factor (TNF)-alpha by cells treated with the extract were measured using enzyme-linked immunosorbent assay. Results: The extract exhibited cytotoxic effects against all the cancer cell lines used but HeLa and Daudi were the most sensitive cells, with IC50 values of 63.18 pg/mL and 69.27 mu g/mL, respectively. Selective cytotoxicity was observed between the HeLa and normal BEAS-2B cell lines. The extract arrested the cell cycle at the S and G2 phases. In addition, apoptotic cell death was detected in HeLa and A549 cells. Moreover, the plant extract caused a significant decrease in VEGF secretion in A549 cells and a fluctuation in IL-1 alpha, IL-6, and TNF-alpha secretion in A549 and Daudi cells. Conclusion: These observations suggest that the flowering parts of C. solstitialis may be a potential source in the development of natural drugs for the treatment of cancer and modulation of cytokine secretion.en_US
dc.description.sponsorshipScientific Research Projects (BAP) of Mugla Sitki Kocman University [15/006, 15/247]en_US
dc.description.sponsorshipThis work was supported by grants 15/006 and 15/247 from Scientific Research Projects (BAP) of Mugla Sitki Kocman Universityen_US
dc.item-language.isoengen_US
dc.publisherTurkish Pharmacists Assocen_US
dc.item-rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCentaurea Solstitialisen_US
dc.subjectCancer Cell Linesen_US
dc.subjectAnti-Canceren_US
dc.subjectAnti-Inflammatoryen_US
dc.titleThe Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Linesen_US
dc.item-typearticleen_US
dc.contributor.departmentMÜ, Fen Fakültesi, Moleküler Biyoloji Ve Genetik Bölümüen_US
dc.contributor.authorID0000-0001-5191-365X
dc.contributor.institutionauthorAlper, Mehlika
dc.contributor.institutionauthorGüneş, Hatice
dc.identifier.doi10.4274/tjps.galenos.2018.27146
dc.identifier.volume16en_US
dc.identifier.issue3en_US
dc.identifier.startpage273en_US
dc.identifier.endpage281en_US
dc.relation.journalTurkish Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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