Kimya Bölümü Koleksiyonu

Copper based metal organic framework decorated with gold nanoparticles as a new electrochemical sensor material for the detection of L-Cysteine in milk samples

Sun, 01 Jan 2023 00:00:00 GMT

Copper based metal organic framework decorated with gold nanoparticles as a new electrochemical sensor material for the detection of L-Cysteine in milk samples Perk, Benay; Tepeli-Büyüksünetci, Yudum; Anık, Ülkü A facile electrochemical sensor based on carbon felt electrode (CFE) modified with gold nanoparticles decorated copper based metal organic framework (AuNPs@Cu-MOF) was achieved for the electrochemical sensing of L-Cysteine (L-Cys). For this purpose, AuNPs@Cu-MOF was synthesized and characterized. The electrochemical behaviors of L-Cys at plain and modified CFEs were investigated via cyclic voltammetry (CV). According CV results, AuNPs@Cu-MOF structure showed a catalytic effect on the oxidation of L-Cys as well as increasing the active electrode surface area by 206% compared to bare CFE. In addition, the pH effect on electrochemical determination of L-Cys at AuNPs@Cu-MOF/CFE was widely examined, and it was determined that the best oxidation peak current of L-Cys was obtained in pH 5 acetate buffer. Moreover, a linear detection range of 30–400 µM for L-Cys with a limit of detection value of 2.21 µM (n = 3) was achieved with the proposed electrochemical sensor. The developed L-Cys sensor was also applied for L-Cys detection in various milk samples and acceptable recovery values were obtained ranging from 100.05 to 108.45%.

Adsorptive Removal of Oxytetracycline Using Polymer Coated Magnetic Nanoparticular Activated Carbon: Synthesis, Characterization and Adsorption Isotherms and Kinetics Studies

Sun, 01 Jan 2023 00:00:00 GMT

Adsorptive Removal of Oxytetracycline Using Polymer Coated Magnetic Nanoparticular Activated Carbon: Synthesis, Characterization and Adsorption Isotherms and Kinetics Studies Uğurlu, Mehmet; Osman, Hüseyn; Vaizoğullar, Ali İmran In the presented study, the removal of oxytetracycline (OTC) from aqueous solution by adsorption was investigated onto Active Carbon (AC), Magnetic activated carbon (MagAC), Styrene-Butadiene-Styrene Magnetic Activated Carbon (SBS/MagAC) and poly charbon magnetic activated carbon (PC/MagAC). The process optimization was carried out by investigating the effects of pH, temperature, solid-liquid ratio, adsorbent type, and initial concentrations. The data showed that adsorption reached equilibrium in as little as one hour. less adsorption at low pH values and more at approximately 5.0 values. However, all the materials performed well at room temperature when the situation is examined in terms of kinetics. It was also observed that AC, MagAC, and PC/MagAC are more effective than SBS/MagAC and the initial concentration decreased from 100 ppm to 20 ppm with adsorbents. In addition, at lower concentrations, when 25 ppm and 50 ppm were used, it was observed to 2.5 ppm and 5.0 ppm values. The kinetic results presented that the pseudo-second-order model (r2 ⩾ 0.99) was more effective than that of the pseudo-first-order model (r2 < 0.90). Also, the Intra-particle kinetic model in the adsorption process exhibited two different stages with the diffusion of inter-particle and external diffusion. Adsorption isotherms for all adsorbents were fitted to Langmuir models more effectively than Freundlich models (r2 ⩾ 0.99). Thermodynamics parameters were also calculated. It is seen that OTC can be removed more easily from the aqueous medium by using magnetic and polymeric materials.

Synthesis, Structural Investigations, DNA/BSA Interactions, Molecular Docking Studies, and Anticancer Activity of a New 1,4-Disubstituted 1,2,3-Triazole Derivative

Sun, 01 Jan 2023 00:00:00 GMT

Synthesis, Structural Investigations, DNA/BSA Interactions, Molecular Docking Studies, and Anticancer Activity of a New 1,4-Disubstituted 1,2,3-Triazole Derivative Göktürk, Tolga; Sakallı Çetin, Esin; Hökelek, Tuncer; Pekel, Hanife; Şensoy, Özge; Aksu, Ebru Nur We report herein a new 1,2,3-triazole derivative, namely, 4-((1-(3,4-dichlorophenyl)-1H-1,2,3-triazol-4-yl)methoxy)-2-hydroxybenzaldehyde, which was synthesized by copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The structure of the compound was analyzed using Fourier transform infrared spectroscopy (FTIR), 1H NMR, 13C NMR, UV-vis, and elemental analyses. Moreover, X-ray crystallography studies demonstrated that the compound adapted a monoclinic crystal system with the P21/c space group. The dominant interactions formed in the crystal packing were found to be hydrogen bonding and van der Waals interactions according to Hirshfeld surface (HS) analysis. The volume of the crystal voids and the percentage of free spaces in the unit cell were calculated as 152.10 Å3 and 9.80%, respectively. The evaluation of energy frameworks showed that stabilization of the compound was dominated by dispersion energy contributions. Both in vitro and in silico investigations on the DNA/bovine serum albumin (BSA) binding activity of the compound showed that the CT-DNA binding activity of the compound was mediated via intercalation and BSA binding activity was mediated via both polar and hydrophobic interactions. The anticancer activity of the compound was also tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using human cell lines including MDA-MB-231, LNCaP, Caco-2, and HEK-293. The compound exhibited more cytotoxic activity than cisplatin and etoposide on Caco-2 cancer cell lines with an IC50 value of 16.63 ± 0.27 μM after 48 h. Annexin V suggests the induction of cell death by apoptosis. Compound 3 significantly increased the loss of mitochondrial membrane potential (MMP) levels in Caco-2 cells, and the reactive oxygen species (ROS) assay proved that compound 3 could induce apoptosis by ROS generation.

Assessing the Acute Toxicological Effects of Annona muricata Leaf Ethanol Extract on Rats: Biochemical, Histopathological, and Metabolomics Analyses

Sun, 01 Jan 2023 00:00:00 GMT

Assessing the Acute Toxicological Effects of Annona muricata Leaf Ethanol Extract on Rats: Biochemical, Histopathological, and Metabolomics Analyses Zubaidi, Siti Norliyana; Qadi, Wasim S. M.; Maarof, Syahida; Hellal, Khaoula Annona muricata is a common plant used in Africa and South America to manage various types of disease. However, there is insufficient toxicological information or published standard available regarding repeated dose animal toxicity data. As part of the safety assessment, we exposed Sprague Dawley rats to an acute oral toxicity of A. muricata. The intent of the current study was to use advanced proton nuclear magnetic resonance (H-1 NMR) in serum and urinary metabolomics evaluation techniques to provide the in vivo acute toxicological profile of A. muricata leaf ethanol extract in accordance with the Organization for Economic Co-operation and Development's (OECD) 423 guidelines. A single 2000 mg/kg dose of A. muricata leaf ethanol extract was administered to Sprague Dawley rats over an observational period of 14 days. The toxicity evaluation (physical and behavior observation, body weight, renal function test, liver function test and H-1 NMR analysis) showed no abnormal toxicity. Histopathological analysis manifested mild changes, i.e., the treated kidney manifested mild hypercellularity of mesangial cells and mild red blood cell congestion. In addition, there was mild hemorrhage into tissue with scattered inflammatory cells and mild dilated central vein with fibrosis in the liver. However, the changes were very mild and not significant which correlate with other analyses conducted in this study (biochemical test and H-1 NMR metabolomic analysis). On the other hand, urinary H-1 NMR analysis collected on day 15 revealed high similarity on the metabolite variations for both untreated and treated groups. Importantly, the outcomes suggest that A. muricata leaf ethanol extract can be safely consumed at a dose of 2000 mg/kg and the LD50 must be more than 2000 mg/kg.