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dc.contributor.authorKıyıkçı, Hüseyin Furkan
dc.contributor.authorAvcı, Okan
dc.contributor.authorTepeli Büyüksünetçi, Yudum
dc.contributor.authorTimur, Suna
dc.contributor.authorAnık, Ülkü
dc.date.accessioned2022-12-21T08:31:11Z
dc.date.available2022-12-21T08:31:11Z
dc.date.issued2022en_US
dc.identifier.citationKıyıkçı HF, Avcı O, Tepeli Büyüksünetçi Y, Timur S, Anık Ü. Oxidase mimicking Co/2Fe MOF included biosensor for sialic acid detection. Talanta. 2022 Dec 5;254:124166. doi: 10.1016/j.talanta.2022.124166. Epub ahead of print. PMID: 36493566.en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2022.124166
dc.identifier.urihttps://hdl.handle.net/20.500.12809/10444
dc.description.abstractA facile amperometric biosensor that included oxidase mimicking Co/2Fe metal-organic framework (MOF) for sialic acid (SA) detection was prepared. Amperometric SA biosensor was constructed on a gold screen-printed electrode via immobilization of Co/2Fe MOF and N-acetylneuraminic Acid Aldolase (NANA-Aldolase) enzyme, respectively. NANA-Aldolase enzyme converts free SA into pyruvate and N-acetyl-D-mannosamine. After this conversion, oxidase mimicking Co/2Fe bimetallic MOF converts pyruvate into acetylphosphate and O2 into H2O2. Investigation of analytical characteristics resulted with the linear range of 0.02 mM–1.00 mM of SA concentration with limit of detection value of 0.026 mM. Sample application studies with developed SA biosensor were carried out with GD3 ganglioside and HeLa cancer cell lines which have high SA concentrations while A549 cell lines were also used as control group. Before detecting free SA, the bound SA was freed from SA sources where every step was monitored via electron impedance spectroscopy. Then, free SA was successfully detected with the amperometric SA biosensor and as a result, more practical and accurate system was developed.en_US
dc.item-language.isoengen_US
dc.publisherElsevier B.V.en_US
dc.relation.isversionof10.1016/j.talanta.2022.124166en_US
dc.item-rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlycanen_US
dc.subjectSialic aciden_US
dc.subjectElectrochemical biosensoren_US
dc.subjectCo/2Fe bimetallic MOFen_US
dc.subjectOxidase mimickingen_US
dc.titleOxidase mimicking Co/2Fe MOF included biosensor for sialic acid detectionen_US
dc.item-typearticleen_US
dc.contributor.departmentMÜ, Fen Fakültesi, Kimya Bölümüen_US
dc.contributor.authorID0000-0002-5095-8829en_US
dc.contributor.authorID0000-0002-4717-7933en_US
dc.contributor.authorID0000-0002-3607-7208en_US
dc.contributor.institutionauthorKıyıkçı, Hüseyin Furkan
dc.contributor.institutionauthorAvcı, Okan
dc.contributor.institutionauthorTepeli Büyüksünetçi, Yudum
dc.contributor.institutionauthorTimur, Suna
dc.contributor.institutionauthorAnık, Ülkü
dc.identifier.volume254en_US
dc.relation.journalTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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