Genetic Analyses of Some Central Anatolian Domestic Duck Populations with Inter Simple Sequence Repeat (ISSR): A Preliminary Study
Özet
The aim of this study is to investigate the genetic structure of some domestic duck populations from Kirsehir and Yozgat provinces of the Central Anatolia region of Turkey. Blood samples were obtained from the venae cutenea ulnaris of 76 ducks from four different locations. Eleven ISSR primers produced 73 reproducible and bright bands. The number of polymorphic loci was 72 and the percentage of polymorphic loci was 98.6%. Gene diversity (H-T) in total population and magnitude of differentiation among populations (G(ST)) were 0.198 and 0.183, respectively. The genetic distances between regions under investigation were found among 0.0157 and 0.0991. Analysis of molecular variance (AMOVA) reflected that 83% of within variation and 17% among population variation. First three given values of principle coordinate analysis explained 69.8% of total variation as 45.6, 13.8 and 10.4%, respectively. The dendrogram showed two main branches: one contains Kirsehir (L1, L2, L3), the other includes Yozgat region (L4). Shannon's Information index (I) value was 0. 331. The gene flow (Nm) among populations was analyzed and Nm value was estimated as 2.23 with the low level of differentiation among populations. The results indicated that genetic variations of some Central Anatolian domestic duck populations are determined using ISSR marker and might provide information for future breeding strategies.
