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dc.contributor.authorTopçular, Ceren
dc.contributor.authorAyhan, Hakan
dc.date.accessioned2020-11-20T17:51:01Z
dc.date.available2020-11-20T17:51:01Z
dc.date.issued2008
dc.identifier.issn1303-5002
dc.identifier.issn2687-475X
dc.identifier.urihttps://app.trdizin.gov.tr//makale/TVRBME1EQXhNUT09
dc.identifier.urihttps://hdl.handle.net/20.500.12809/8661
dc.description.abstractIn this study, cross-linked Horseradish Peroxidase (HRP) aggregates were prepared. Bovin Serum Albumin (BSA), a proteic feeder, and glutaraldehyde, a bifunctional reactive, a crosslinker agent has also been used. The initial enzyme concentration (0.05 mg/ml), BSA (5 mg) and glutaraldehyde (GA) (4 & #956; l/ml) amounts were optimized and maximum immobilized activities were obtained. The kinetic parameters of free and immobilised enzyme have been calculated by using Lineweaver-Burk plots. The saturation constant (Km) and the maximum reaction rate (Vm) of free enzyme were found as 2.33 mM and 0.670mmol/l.sec, respectively. The apparent kinetic parameters ($Km _{app}$ and $Vm _{app}$) of cross linked enzyme aggregates have been calculated as 33.4 mM and 0.127 mmol/l.sec, respectively.en_US
dc.item-language.isoengen_US
dc.item-rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCross-linked enzyme aggregatesen_US
dc.titleCarrier free cross-linked peroxidase aggregates: synthesis and characterizationen_US
dc.item-typearticleen_US
dc.contributor.departmentMÜ, Fen Fakültesi, Kimya Bölümüen_US
dc.contributor.institutionauthorAyhan, Hakan
dc.identifier.volume36en_US
dc.identifier.issue3en_US
dc.identifier.startpage255en_US
dc.identifier.endpage261en_US
dc.relation.journalHacettepe Journal of Biology and Chemistryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanen_US


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