Zootekni ve Hayvan Besleme Bölümü Koleksiyonu

Evaluation of Peppermint (Mentha piperita L.) Essential Oil as a Digestive Tract Regulator in Broilers

2023-10-04T08:32:03Z

Evaluation of Peppermint (Mentha piperita L.) Essential Oil as a Digestive Tract Regulator in Broilers Bülbül, Tuba; Özdemir, Vural; Bülbül, Aziz The aim of this study was to investigate the effects of peppermint (Mentha piperita L.) essential oil on duodenal and colonic contractions of broilers in vitro. For this purpose, 10 broiler intestines (42 days old) obtained from a slaughterhouse were brought to the laboratories by immersed in Krebs solution (NaCl, 118 mmol/L; KCl, 4.7 mmol/L; CaCl2, 2.5 mmol/L; MgSO4, 1 mmol/L; KH2 PO4, 1 mmol/L; glucose, 11 mmol/L; NaHCO3, 25 mmol/L) at +4°C. Isolated duodenal and colon tissue strips were placed in a four-channel, isolated organ bath system, where they were exposed to Krebs solution aerated with a gas mixture of 95% O2-5% CO2, at 39°C. The effect of peppermint essential oil (PEO) obtained by hydrodistillation method on spontaneous duodenum and colon contractions was evaluated in the concentration range of 0.1-1000 μg/mL. Subsequently, the effects of 300 and 1000 μg/mL PEO were reevaluated on acetylcholine-induced contractions after incubation with Nω-Nitro-L-arginine (L-NNA, NOS inhibitor) and Methylene Blue (MB, cGC inhibitor). It was determined that the PEO dose-dependently decreased the amplitude of spontaneous and acetylcholine-induced contractions in both isolated tissues. This relaxant effect persisted after L-NNA and MB incubations. In conclusion, PEO induced relaxation in isolated duodenum and colon intestinal smooth muscles not through the nitric oxide-sGMP pathway.

Effects of Dietary Sodium Nitroprusside and NG-Nitro-L-Arginine Methyl Ester on Growth Performance, Some Organs Development Status and Immune Parameters in Broilers

2023-05-22T11:34:13Z

Effects of Dietary Sodium Nitroprusside and NG-Nitro-L-Arginine Methyl Ester on Growth Performance, Some Organs Development Status and Immune Parameters in Broilers Bülbül, Tuba; Sefergil, Şamil; Bülbül, Aziz This study was conducted to determine the effects of dietary supplementation of sodium nitroprusside (SNP), a nitric oxide (NO) donor, and NG-nitro-L-arginine methyl ester (L-NAME), an NO synthase inhibitor, on growth performance, organ development, and immunity in broilers. A total of 560 one-day-old mixed-gender broiler chickens (ROSS 308) were divided into one control and seven experimental groups. The experimental groups were fed a basal diet supplemented with 25, 50, 100, and 200 ppm SNP, and 25, 50, and 100 ppm L-NAME in the starter and grower diets. Body weight gain increased in groups receiving 25-100 ppm L-NAME on day 21 and 100 ppm L-NAME on days 0-42. Feed intake increased in the group receiving 100 ppm L-NAME on all days. The feed conversion ratio improved in the group receiving 25 ppm L-NAME on days 0-21, whereas it worsened in groups with 100 and 200 ppm SNP on days 0-42. Serum antibody titers decreased in the 100 ppm L-NAME group on day 21. In conclusion, the supplementation of the NO synthase inhibitor L-NAME to the broilers' diet had a positive effect on the performance parameters, whereas the NO donor SNP worsened these parameters, especially on days 0-21.

BIOCHEMICAL, HEMATOLOGICAL AND HISTOPATHOLOGICAL EVALUATION OF THE FOOD-SAFETY OF THE LEAF EXTRACT OF PAPAVER SOMNIFERUM IN RATS

2021-08-26T07:45:54Z

BIOCHEMICAL, HEMATOLOGICAL AND HISTOPATHOLOGICAL EVALUATION OF THE FOOD-SAFETY OF THE LEAF EXTRACT OF PAPAVER SOMNIFERUM IN RATS Bülbül, Tuba; Gür, Emre; Bozkurt, Fatih; Eryavuz, Abdullah; Bülbül, Aziz The fresh shoots of Papaver somniferum L. (opium poppy) are used as a flavouring by the local population in the regions where it is grown. The aim of this research was to determine the food-safety of the extract obtained from the fresh leaves of Papaver somniferum L. plant. The extract of the leaves of the opium poppy plant, were dissolved in 1 ml of drinking water and administered to healthy male rats, in 4 different doses (400, 800, 1600 and 2000 mg/kg body weight/day) on a single day to evaluate the acute and each day for 30 days to evaluate the subacute effect. Blood was withdrawn for hematological and biochemical analysis. Lung, heart, liver and kidney tissues were taken for pathological examination. It was observed that the ALT enzyme decreased at the dose of 800, 1600 and 2000 mg/kg body weight/day; furthermore, AST, GGT and CK-MB enzymes decreased at the doses of 1600 and 2000 mg/kg body weight/day in the subacute phase (p<0.05). It can be stated that the water extract obtained from the leaves of Papaver somniferum L. has no negative effect on the biochemical, hematological and pathological parameters in the given doses, furthermore the extract may have a protective effect on the liver tissue.

Effect of myrtle (Myrtus communisL.) essential oil on oxidant-antioxidant balance in rats with propylthiouracil-induced hypothyroidism

2021-04-12T20:42:44Z

Effect of myrtle (Myrtus communisL.) essential oil on oxidant-antioxidant balance in rats with propylthiouracil-induced hypothyroidism Atik, Hülya; Bülbül, Tuba; Özdemir, Vural; Avcı, Gülcan; Bülbül, Aziz Among today's health problems, metabolic diseases are at the forefront. Hypothyroidism (HT) is a disease characterized by increased TSH, decreased T3&T4 concentrations in serum, with overall metabolic slowdown. Although there are many studies in the literature about oxidative status in HT, statements in these studies are contradictory. In our study, the effect of essential oils obtained from the leaves, flowers, and roots ofMyrtus communisL. on oxidative metabolism in an HT model induced by propylthiouracil (PTU) in rats was investigated. A total of 36 Wistar albino rats were randomly divided into six groups as follows: (1) Control, (2) PTU, (3)M. communisL. oil 200 (MO 200), (4)M. communisL. oil 400 (MO 400), (5) PTU + MO 200, and (6) PTU + MO 400. In our study, while oxidative status deteriorates in groups given PTU, antioxidant activity increases in groups givenM. communisL. oil. Practical applications Essential oils are aromatic oily liquids derived from different parts of plants.M. communisL. is one of the best-known herbs in the class of aromatic and medicinal plants. This paper emphasizes the effect ofM. communisL. oil on the negative oxidative state that occurs in HT conditions. The present study provides a positive effect of essential oils obtained from theM. communisL. on the oxidative state seen in HT. In light of this information, it may be beneficial to useM. communisL. oil due to its antioxidative effect in HT conditions. WOS: 000574818600001; PubMed ID: 33015880