Graphene-metallic nanocomposites as modifiers in electrochemical glucose biosensor transducers

Abstract

Graphene sheets and three different graphene-metallic nanocomposites including graphene-copper (graphene-Cu), graphene-nickel (graphene-Ni) and graphene-platinum (graphene-Pt) were prepared and characterized in the first place. Then the electrochemical performances of these nanocomposites were tested in glucose biosensor transducers, which were formed by combining these metallic nanocomposites with glucose oxidase enzyme and glassy carbon paste electrode (GCPE). This is the first work that includes the usage of these graphene-Me nanocomposites as a part of glucose biosensor transducer. Fabricated amperometric biosensors linear ranges were obtained as follow: For the plain graphene, the linear range was found in the concentration range between 50 mu Mand 800 mu Mwith the RSD (n= 3 for 50 mu M glucose) value of 12.86% and LOD value of 7.2 mu M. For graphene-Pt modified glucose biosensor, the linear range was between 10 mu M and 600 mu M with the RSD (n= 3 for 50 mu M glucose) value of 3.45% and LOD value of 3.06 mu M. In the case of graphene-Ni modified glucose biosensor, the values were 25 mu M to 600 mu M with the RSD (n= 3 for 50 mu M glucose) value of 8.76% and LOD value of 24.71 mu M and for graphene-Cu modified glucose biosensor linear range was 25 mu M to 400 mu M with the RSD (n= 3 for 50 mu M glucose) value of 3.93% and LOD value of 2.87 mu M.