Anti C-reactive protein immobilization onto poly(glycidyl methacrylate) microspheres and its use for diagnosis

Abstract

A method for a polymeric carrier based test was developed for the diagnosis of C-reactive protein (CRP) as an alternative to commercial kits. Poly(glycidyl methacrylate) (PGMA) particles were synthesized by dispersion polymerization method from the glycidyl methacrylate (GMA) monomer. The monomer transformation value was 88.67% by weight. In the first step the epoxide rings were opened to form amine groups on the particle surfaces. The optimized conditions were found for the antigen to be immobilized onto the surface-modified microspheres. Agglutination tests were carried out under in vitro conditions with the anti-human CRP immobilized microspheres with samples from patient serum. Relatively high sensitivity was obtained with a short reaction time between doing the test after receiving patient serum compared with the selected commercial kit now in use. By referring to the agglutination intensity scores, using only 10 mg/L samples of plasma, the CRP concentration was detectable with these new kits.