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dc.contributor.authorÇatav, Şükrü Serter
dc.contributor.authorElgin, Emine Sonay
dc.contributor.authorDağ, Çağdaş
dc.contributor.authorStark, Jaime L.
dc.contributor.authorKüçükakyüz, Köksal
dc.date.accessioned2020-11-20T14:49:45Z
dc.date.available2020-11-20T14:49:45Z
dc.date.issued2018
dc.identifier.issn1573-3882
dc.identifier.issn1573-3890
dc.identifier.urihttps://doi.org/10.1007/s11306-018-1440-y
dc.identifier.urihttps://hdl.handle.net/20.500.12809/1321
dc.description0000-0002-9934-254Xen_US
dc.descriptionWOS: 000447392600001en_US
dc.descriptionPubMed ID: 30830436en_US
dc.description.abstractIntroductionIt is well known that plant-derived smoke stimulates seed germination and seedling growth in many plants. Although a number of transcriptomics and proteomics studies have been carried out to understand the mode of action of smoke, less is known about the biochemical alterations associated with smoke exposure in plants.ObjectivesThe aims of this study were (1) to determine the metabolic alterations in maize roots pre-treated with various concentrations of smoke solution, and (2) to identify the smoke-responsive metabolic pathways during early root growth period.MethodsMaize seeds were pre-treated with different concentrations of smoke solutions for 24h and then grown for 10days. 600-MHz H-1 NMR spectroscopy was performed on the aqueous root extracts of maize seedlings. The metabolite data obtained from the NMR spectra were analyzed by several statistical and functional methods, including one-way ANOVA, PCA, PLS-DA and pathway analysis.ResultsOur study identified a total of 29 metabolites belonging to various chemical groups. Concentrations of 20 out of these 29 metabolites displayed significant (p<0.05) changes after at least one smoke pre-treatment compared to thecontrol. Moreover, functional analyses revealed that smoke pre-treatments markedly affected the carbohydrate- and energy-related metabolic pathways, such as galactose metabolism, glycolysis, glyoxylate metabolism, tricarboxylic acid cycle, and starch/sucrose metabolism.ConclusionsTo our knowledge, this is the first study that investigates smoke-induced biochemical alterations in early root growth period using NMR spectroscopy. Our findings clearly indicate that smoke either directly or indirectly influences many metabolic processes in maize roots.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Mugla Sitki Kocman UniversityMugla Sitki Kocman University [13/02, 15/153]; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [P41GM103399, S10RR02781, S10RR08438, S10RR023438, S10RR025062, S10RR029220]; University of Wisconsin-Madison; NSFNational Science Foundation (NSF) [DMB-8415048, OIA-9977486, BIR-9214394]; USDAUnited States Department of Agriculture (USDA); NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399, P41GM103399] Funding Source: NIH RePORTERen_US
dc.description.sponsorshipThis work is a part of Ph.D. thesis of the first author, funded by the Scientific Research Projects Coordination Unit of Mugla Sitki Kocman University (Grant Numbers: 13/02 and 15/153). This study made use of the National Magnetic Resonance Facility at Madison, which is supported by NIH Grant P41GM103399 (NIGMS), old number: P41RR002301. Equipment was purchased with funds from the University of Wisconsin-Madison, the NIH (P41GM103399, S10RR02781, S10RR08438, S10RR023438, S10RR025062, S10RR029220), the NSF (DMB-8415048, OIA-9977486, BIR-9214394), and the USDA.en_US
dc.item-language.isoengen_US
dc.publisherSpringeren_US
dc.item-rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSmokeen_US
dc.subjectRoot Growthen_US
dc.subjectNMR Spectroscopyen_US
dc.subjectMetabolomicsen_US
dc.subjectMaizeen_US
dc.titleNMR-based metabolomics reveals that plant-derived smoke stimulates root growth via affecting carbohydrate and energy metabolism in maizeen_US
dc.item-typearticleen_US
dc.contributor.departmentMÜ, Fen Fakültesi, Biyoloji Bölümüen_US
dc.contributor.departmentMÜ, Fen Fakültesi , Kimya Bölümü
dc.contributor.institutionauthorÇatav, Şükrü Serter
dc.contributor.institutionauthorElgin, Emine Sonay
dc.contributor.institutionauthorKüçükakyüz, Köksal
dc.identifier.doi10.1007/s11306-018-1440-y
dc.identifier.volume14en_US
dc.identifier.issue11en_US
dc.relation.journalMetabolomicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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