Role of Leptin (rs7799039) and Leptin Receptor (rs1137101) Gene Polymorphisms in the Development of Uterine Leiomyoma

Abstract

Objective: To evaluate whether there is an association between leptin (rs7799039) and leptin receptor (rs1137101) gene polymorphisms and risk of Uterine Leiomyoma (ULM) development Design: Controlled prospective study Setting: Department of Obstetrics and Gynecology and Medical Biology School of Medicine, Mugla Sitki Kocman University, Mugla, Turkey Subjects: This cross-sectional, clinical study included 103 perimenopausal patients who had ULM and 82 age-matched healthy perimenopausal controls. Serum estradiol (E29, Follicule Stimulating Hormone FSH) and hemoglobin levels were measured. Intervention: Leptin and leptin receptor gene polymorphisms were determined by using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) methods. Main outcome measures : Genotype of leptin gene Results: Median FSH level was significantly higher in the control group (62.13 Vs 32.46; p < 0.001), whereas Median E2 level was higher in the ULM group (114.94 Vs. 29.08; p < 0.0019). According to genotype and alleles analyzing, GA genotype of leptin rs7799039 polymorphism (p = 0.004, OR = 0.380, 95% CI = 0.173 - 0.837), AA genotype of leptin receptor rs1137101 polymorphism (p = 0.020, OR = 0.326 CI = 0.144 - 0.736) and A Allele of leptin receptor rs1137101 polymorphism (p = 0.003, OR = 0.537, 95% CI = 0.346-0.803) presented protective effects for ULM development. In correlation analysis, AA genotype of leptin gene polymorphism was found to be significantly related with increased E2 levels in ULM patients (p = 0.032) but not in control group. Conclusion: Leptin and leptin receptor gene polymorphisms together with increased estrogen levels might affect susceptibility to ULM development.