| dc.contributor.author | Anık, Ülkü | |
| dc.contributor.author | Cubukcu, Meliha | |
| dc.contributor.author | Ertas, F. Nil | |
| dc.date.accessioned | 2020-11-20T15:02:30Z | |
| dc.date.available | 2020-11-20T15:02:30Z | |
| dc.date.issued | 2016 | |
| dc.identifier.issn | 2169-1401 | |
| dc.identifier.issn | 2169-141X | |
| dc.identifier.uri | https://doi.org/10.3109/21691401.2015.1008504 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12809/2521 | |
| dc.description | Ertas, Fatma/0000-0001-8982-9621; anik, ulku/0000-0002-3607-7208; | en_US |
| dc.description | WOS: 000376136500028 | en_US |
| dc.description | PubMed ID: 25697045 | en_US |
| dc.description.abstract | A biosensor was developed using glutathione peroxidase (GSH-Px) as the enzyme. Firstly, platinum (Pt) nanoparticles were deposited onto a glassy carbon paste electrode (GCPE), and then GSH-Px was immobilized by means of gelatin that was then crosslinked with glutaraldehyde. The measurement was based on the electrochemical oxidation of GSH to its disulfide form in the presence of hydrogen peroxide. The linear range was found to be between 10 and 250 M, with a correlation coefficient of R-2 = 0.9968. The R.S.D value for 25 M GSH (n = 6) was calculated as 2.92%. Finally, the proposed biosensor was used to analyze GSH in a synthetically prepared plasma sample, and a promising recovery value was obtained. | en_US |
| dc.description.sponsorship | Scientific and Technical Research Council of Turkiye (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109T885]; Ege UniversityEge University [2011 FEN 088] | en_US |
| dc.description.sponsorship | This work is supported by The Scientific and Technical Research Council of Turkiye (TUBITAK) (project no. 109T885). M.C. and F.N.E are gratefully acknowledged the grant from Ege University, Project number 2011 FEN 088. | en_US |
| dc.item-language.iso | eng | en_US |
| dc.publisher | Taylor & Francis Ltd | en_US |
| dc.item-rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Biosensor | en_US |
| dc.subject | Glassy Carbon Paste Electrode | en_US |
| dc.subject | Glutathione | en_US |
| dc.subject | Glutathione Peroxidase | en_US |
| dc.subject | Pt Nanoparticles | en_US |
| dc.title | An effective electrochemical biosensing platform for the detection of reduced glutathione | en_US |
| dc.item-type | article | en_US |
| dc.contributor.department | MÜ | en_US |
| dc.contributor.departmentTemp | [Anik, Ulku; Cubukcu, Meliha] Mugla Sitki Kocman Univ, Fac Sci, Dept Chem, Kotekli, Mugla, Turkey -- [Cubukcu, Meliha; Ertas, F. Nil] Ege Univ, Fac Sci, Dept Chem, Izmir, Turkey | en_US |
| dc.identifier.doi | 10.3109/21691401.2015.1008504 | |
| dc.identifier.volume | 44 | en_US |
| dc.identifier.issue | 3 | en_US |
| dc.identifier.startpage | 971 | en_US |
| dc.identifier.endpage | 977 | en_US |
| dc.relation.journal | Artificial Cells Nanomedicine and Biotechnology | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
