Effects of Seminal Plasma Properties on Percentage and Duration of Shabut (Barbus grypus Heckel, 1843) Sperm Motility

Abstract

The aim of this study was to demonstrate the effects of seminal plasma properties (Ca2+, Na+, K+ and Mg2+, glucose, urea, triglyceride, total protein concentrations, pH and osmolality) on progressive sperm motility percentage and duration in Barbus grypus. Osmolality (mOsmol/kg) ranged from 81-263, while ionic contents (mM) varied between 20-79 Na+, 1.2-58.4 K+, 0.50-1.05 Ca2+, and 0.51-1.44 Mg2+ respectively. Spermatozoa density was 15.12 +/- 1.11 x10(9)/mL, semen volume 3.87 +/- 0.41 mL while total protein, urea triglyceride and glucose were 0.60 +/- 0.17 mg/mL, 4.08 +/- 0.08 mg/dL, 8.42 +/- 0.62 mg/dL, 5.17 +/- 0.11 mg/dL respectively. The data obtained allows comparison of variations in semen parameters and enables us to determine how these parameters relate to motility characteristics. The groups formed by hierarchical cluster analysis (CA) based on the semen properties corresponded to sperm motility characteristics. According to principal component analysis (PCA) results, osmolality, protein concentration, Na+ and K+ were strongly correlated with motility characteristics and were responsible for total variation in data compared to other parameters. Multivariate statistical methods as well as ionic ratios were used to analyze the data.