ASSESSING INTRA-SPECIFIC VARIATION WITHIN BACILLUS SPP. STRAINS USING DNA FINGERPRINTING

Abstract

Bacillus subtilis strains are known to be prominent biologics playing different roles both in biological degradation of organic compounds and bio-control of plant diseases. In the present study, a schematic diagram was improved using efficient ISSR markers, selected in previous screening studies and suggested in view of their cost and labor-effective roles in discrimination of Bacillus spp. strains. To assess their efficiency and use in diversification of Bacillus spp. strains, whole findings were compared with 16S rDNA analysis. The results by selected Inter-Simple Sequence Repeats (ISSR) markers showed convenient, highly reproducible results to be a tool for revealing genetic relationships in Bacillus spp. strains, with regard to 16S rDNA. Even ISSRs have no informative knowledge based on gene sequence. Quick studies on structure and dynamics of microbial communities in ecosystems are of importance. Therefore, ISSR fingerprinting is suggested to be an efficient tool in intraspecific discrimination of Bacillus subtilis strains in further studies.