Enzyme-based scavengers and lipid peroxidation in some wild edible Agaricales s.l. mushrooms from Mugla (Turkey)

Abstract

Antioxidant enzyme activities, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and lipid peroxidation levels (LPO), were investigated in 10 wild edible Agaricales s.l. mushroom species from four families (Boletaceae, Hygrophoraceae, Lepiotaceae, and Tricholomataceae) from Mugla (Turkey). Highest SOD activities in stipes and pilei were observed in Hygrophorus olivaceoalbus as 10.8 +/- 3.0 and 8.0 +/- 3.0 IU/mg protein, respectively. Maximum CAT activity was found in the stipe of Lepiota mastoidea as 13.5 +/- 1.5 IU/mg protein. The other peroxidase, GSH-Px, was highest in stipes of H. olivaceoalbus as 1.1 +/- 0.2 IU/mg protein. The highest LPO level was found in the stipe of Clitocybe alexandri as 127.0 +/- 8.1 nmol MDA/g wet weight. Correlation analyses showed that there was a significant correlation (r = 0.627, p < 0.05) between SOD and LPO levels in stipes. On the other hand, we also observed a significant correlation between SOD in stipes and GSH-Px in pilei (r = 0.696, p < 0.05) and between CAT in stipes and LPO in pilei (r = 0.885, p < 0.001) of mushrooms. These results may show that whereas LPO levels were effected by SOD activities in stipes, SOD in stipes also affected the GSH-Px activities in pilei.