NMR-based metabolomics reveals that plant-derived smoke stimulates root growth via affecting carbohydrate and energy metabolism in maize

Abstract

IntroductionIt is well known that plant-derived smoke stimulates seed germination and seedling growth in many plants. Although a number of transcriptomics and proteomics studies have been carried out to understand the mode of action of smoke, less is known about the biochemical alterations associated with smoke exposure in plants.ObjectivesThe aims of this study were (1) to determine the metabolic alterations in maize roots pre-treated with various concentrations of smoke solution, and (2) to identify the smoke-responsive metabolic pathways during early root growth period.MethodsMaize seeds were pre-treated with different concentrations of smoke solutions for 24h and then grown for 10days. 600-MHz H-1 NMR spectroscopy was performed on the aqueous root extracts of maize seedlings. The metabolite data obtained from the NMR spectra were analyzed by several statistical and functional methods, including one-way ANOVA, PCA, PLS-DA and pathway analysis.ResultsOur study identified a total of 29 metabolites belonging to various chemical groups. Concentrations of 20 out of these 29 metabolites displayed significant (p<0.05) changes after at least one smoke pre-treatment compared to thecontrol. Moreover, functional analyses revealed that smoke pre-treatments markedly affected the carbohydrate- and energy-related metabolic pathways, such as galactose metabolism, glycolysis, glyoxylate metabolism, tricarboxylic acid cycle, and starch/sucrose metabolism.ConclusionsTo our knowledge, this is the first study that investigates smoke-induced biochemical alterations in early root growth period using NMR spectroscopy. Our findings clearly indicate that smoke either directly or indirectly influences many metabolic processes in maize roots.