ASSOCIATION OF FETUIN-A WITH GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND OXIDATIVE STRESS IN SICKLE CELL ANEMIA

Abstract

Aim: Oxidative stress has crucial effects over vascular pathophysiology of Sickle Cell Anemia (SCA), and contributes atherosclerosis at advanced stages of the disease. In contrast to be acknowledged the possible role of Fetuin-A in the development of atherosclerosis, its association with vascular changes in SCA appears to warrant further investigation. In the study, we aimed to illuminate vascular pathophysiology of SCA via investigating Fetuin-A levels and its relationship with oxidative stress parameters. Material and methods: Fourty SCA patients and and 35 healthy individuals (n: 35) were included in the study all. After analyzing hematological data, Malondialdehyde (MDA), Glutathione Peroxidase (GSH-Px), Superoxide Dismutase (SOD) and Catalase (CAT) were measured throughout preparing hemolysate from samples with EDTA. Fetuin-A was analyzed from serum samples. Results: Fetuin-A (p<0.001) and MDA levels (p<0.001) were measured significantly higher in SCA group. Similarly, G6PD (p<0.001) and SOD (p<0.01) enzyme activities were determined higher in SCA when compared to the control group. A novel positive correlation was found among Fetuin-A, G6PD (r=0.435, p<0.0001) and MDA (r=0.547, p<0.0001). However, there was no statistical significance between the groups for CAT and GSH-Px. ROC analyze suggested 0,97 ng/mL as the cutoff value for Fetuin-A (Area Under the Curve: 0.713, sensitivity: 62%, specificity: 80%, p:0,002) and 0,06 mu mol/gr Hb as the cutoff value for MDA (Area Under the Curve: 0.898, sensitivity: 87 %, specificity: 80%, p:<0,001). Conclusion: Elevated Fetuin-A in patients with SCA and its positive correlations with both MDA and G6PD suggested that Fetuin-A might have an important role for pathophysiology of SCA. Additionally, Fetuin-A may be useful for evaluating severity of disease with high specificity cutoff value as MDA.