Effects of Exercise and Detraining on Genes Involved in the Synthesis of Nitric Oxide, Carbon Monoxide in Spontaneously Hypertensive Rats
Tarih
2015Yazar
Toprak, Emine KılıçErkek, Özgen Kılıç
Mete, Gülçin Abban
Caner, Vildan
Barış, Cansu
Turhan, Gürkan
Bor Küçükatay, Melek
Kuru, Oktay
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Background: We aimed to determine the effects of exercise followed by detraining on systolic blood pressure (SBP), heme
oxygenase 2 (HO-2) expression, and carboxyhemoglobin (COHb) concentration in spontaneously hypertensive
rats (SHR) to explain the role of carbon monoxide (CO) in this process.
Material/Methods: Animals were randomized into exercised and detrained groups. Corresponding sedentary rats were grouped as
Time 1–2. Swimming of 60 min/5 days/week for 10 weeks was applied. Detraining rats discontinued training for an
additional 5 weeks. Gene and protein expressions were determined by real-time PCR and immunohistochemistry.
Results: Aorta HO-2 histological scores (HSCORE) of hypertensive rats were lower, while SBP was higher. Swimming
caused enhancement of HO-2 immunostaining in aorta endothelium and adventitia of SHR. Exercise induced
elevation of blood COHb index in SHR. Synchronous BP lowering effect of exercise was observed. HO-2 mRNA
expression, HSCORE, and blood COHb index were unaltered during detraining, while SBP was still low in SHR.
Conclusions: CO synthesized by HO-2 at least partly plays a role in SBP regulation in the SHR- and BP-lowering effect of exercise.
Regular exercise with short-term pauses may be advised to both hypertensives and individuals who are at risk.