Isolation and screening of novel antibiotic producing Streptomyces from southwest Turkey soils

Abstract

The purpose of this study is to explore secondary metabolites belonging to Streptomyces which show antimicrobial activities on bacteria. For this aim, a total of 234 different Streptomyces isolates were recovered from soil samples taken from areas with various vegetation types in Mugla and its surrounding. All the isolates were screened with spektra-plak method regarding the production of antibacterial metabolites. In this screening process, the target bacteria chosen from pathogens which are 10 Gram positive and 9 Gram negative clinically significant bacterial pathogens including Stenotrophomonas maltophilia and Staphylococcus which are multi resistant to antibiotics. Out of these, 61 isolates exhibited inhibitory activity against at least one of the tested microorganisms. 73.9% isolates produced antibacterial substances against Gram positive bacteria, 47.4% against Gram negative bacteria. The results indicated that 26 isolates were highly active against at least one clinically significant multi-resistant Staphylococcus spp. with an inhibition zone at >= 20 mm.