Gentamicin release from photopolymerized PEG diacrylate and pHEMA hydrogel discs and their in vitro antimicrobial activities

Abstract

Hydrogels based on poly(ethylene glycol)-diacrylate (PEG-DA) and 2-hydroxyethyl methacrylate (HEMA) were polymerized with cross-linking agent ethylene glycol diacrylate (EGDMA) under mild photoinitiating conditions. PEG-DA and HEMA concentrations of disks with 1 +/- 0.3 rum thickness were 30% and 50% w/w and 40% and 60% w/w, respectively. Gentamicin sulphate was incorporated into the hydrogel during photopolymerization and its release kinetics were tested by spectrophotometric method at 255 nm wavelength in phosphate buffer (pH 7.4) and citrate buffer (pH 2.2). The drug release in citrate buffer was faster compared with to phosphate buffer. The release of drug from 40% HEMA containing hydrogel showed Fickian diffusion mechanisms in phosphate buffer (pH 7.4). Antimicrobial efficiency of the samples was tested by agar diffusion method in two different bacterial cultures (Pseudomonas aeruginosa [ATCC 10145], Staphylococcus aureus [ATCC 25923]). Inhibition zone diameter (mm) surrounding each sample was measured after 24 hr incubation of drug loaded disks onto agar plates at 37 degrees C. Inhibition zone formation also confirms that gentamicin sulphate preserves its antimicrobial activity after subjected to photopolymerization conditions.