UPLC-ESI-MS/MS screening, potential of larvicide and antioxidant activity of bioactive compounds in gagea bohemica extracts

Abstract

This work was designed to phenolic compound analysis with UPLC-ESI-MS/MS (Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry), antioxidant and larvicidal (against Musca domestica and Culex pipiens) activities of acetone, methanol and water extracts of different parts (fresh and underground) of G. bohémica extract. The antioxidant activities were determined using six complementary methods (DPPH, ABTS, Phosphomolybdenum, FRAP, CUPRAC assays and ß-Carotene-linoleic acid method). Among extracts, the lowest IC50 value in the DPPH test was obtained from the acetone extract of the underground part (0.652 ± 0.023 jj,g/mL, IC5 0).The determination of contents were evaluated using total phenolics, flavonoid and tannin contents and found maximum values in acetone extract of underground part 6.365 ± 0.064 (mgGAE/g extract), 27.634 ± 0.610 (mg CE/g extract), 22.968 ± 0.073 (mg CE/g extract) respectively. Phenolic compounds of water extracts were determined 32 phenolic compounds using UPLC-ESI-MS/MS, and caffeic acid for both parts was found as the major compound with a high amount. The fresh part is more toxic than the underground part with 60.212 ± 2.778 (1 mg/mL dose, %) against M. domestica, and with 0.190 (mg/mL, LC5 0) against Cx. pipiens. These results about G. bohemica was shown as a source of natural potential antioxidant, and have potential use of pharmaceutical, agricultural industry. © by PSP.