Antimicrobial activities and usage in folkloric medicine of some Lamiaceae species growing in Mugla, Turkey

Abstract

Bu calismada; Lamiaceae familyasina dahil ve geleneksel tedavide kullanilan 23 bitki turu, Mugla'nin farkli yorelerinden toplanmis, kurutulmus ve sokslet ekstraksiyon aparatinda etanol ile ekstrakte edilmistir. Bitki ekstraktlarinin antimikrobiyal aktiviteleri, antibiyotiklere coklu direncli bakterilerin de dahil oldugu cesitli test mikroorganizmalari uzerinde arastirilmistir. Ekstraktlarin antimikrobiyal aktiviteleri disk difuzyon metodu ile belirlenmistir. Test mikroorganizmalari olarak; 7 Gram pozitif, 7 Gram negatif bakteri ve C. albicans secilmistir. Ayrica inhibisyon zonlarinin karsilastirilmasi amaciyla cesitli standart antibiyotik diskleri kullanilmistir. S. verbenaca, T. chamaedrys ssp. lydium, T. divaricatum ssp. villosum, T. polium, S. annua ssp. annua var. annua, S. albiflora, S. leptoclada ve P. vulgaris'e ait etanolik ekstraktlarin antimikrobiyal aktiviteleri, antibiyotiklere coklu direncli Staphylococcus suslarinin da dahil oldugu Gram pozitif bakterilere karsi belirlenmistir. S. leptoclada'ya ait etanolik ekstrakt en yuksek inhibisyon etkisine sahiptir. Buna karsin tum bitkilere ait etanolik ekstraktlar Gram negatif bakteriler ve C. albicans uzerinde etkisizdir. Bakteriler uzerinde inhibisyon etkisi gosteren etanolik ekstraktlar S. aureus ATCC 25923, MU 38, MU 44 ve S. epidermidis uzerinde daha etkilidir. S. leptoclada'nin S. aureus ATCC 25923 uzerindeki inhibisyon zonu ayni bakteri uzerindeki oksasilinin inhibisyon zonundan daha yuksektir.

In this study; 23 selected plant species belonging to the Lamiaceae family, used in traditional treatments, were collected from different localities of Mugla, dried and extracted with ethanol using the soxhlet extraction apparatus. The antimicrobial activities of the plant extracts on the various test microorganisms, including multiple antibiotic resistant bacteria, were investigated. Antimicrobial activities of the extracts were determined by the disc diffusion method. Test microorganisms were; 7 Gram positive, 7 Gram negative bacteria and Candida albicans. Also different standart antibiotic discs were used for comparison for the inhibition zones. The antimicrobial activities of the ethanolic extracts of Salvia verbenaca, Teucrium chamaedrys ssp. lydium, Teucrium divaricatum ssp. villosum, Teucrium polium, Stachys annua ssp. annua var. annua, Sideritis albiflora, Sideritis leptoclada and Prunella vulgaris demonstrated the inhibition effects against Gram positive bacteria including multiple antibiotic resistant Staphylococcus strains. The ethanolic extract of S. leptoclada was the most effective extract. On the contrary, all of the ethanolic extracts were not effective on Gram negative bacteria and C. albicans. The ethanolic extracts of the plants, which inhibited the bacteria, mostly inhibited the growth of Staphylococcus aureus ATCC 25923, MU 38, MU 44 and Staphylococcus epidermidis. Even the inhibition zone of S. leptoclada on S. aureus ATCC 25923 was greater than the inhibition zone of oxacillin on the same bacteria.