Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures

Abstract

Karısık mikrobiyal kültürler Gediz havzasından alınan toprak örneklerinden zenginlestirme ile elde edildi. Fungusid thiram içeren zenginlestirilmis kültürlerden fungus ve bakteri türleri izole edildi ve kısmen tanımlandı. Morfolojik ve biyokimyasal testlere verdikleri cevaplara göre funguslar; Aspergillus niger, A. flavus ve Penicillium steckii, olarak tanımlandı. Bakteriyal izolatlar; Bacillus, Arthrobacter, Moraxella-like Acinetobacter ve Streptomyces genuslarına dahil edildi. Izolatlar 0.2 mg/ml thiram içeren mineral besiyerinde gelistiler ve büyüme faktörlerine ihtiyaç duymazlar.

Mixed microbial cultures were obtained by enrichment from soil samples collected from the Gediz basin. A number of species of bacteria and fungi were isolated and partially characterized from enrichment clutures containing the fungicide thiram. According to their responses in morphological and biochemical tests fungi were identified as Aspergillus niger, A. flavus and Penicilium steckii. Bacterial isolates were assigned to the genera: Bacillus, Arthrobacter, Moraxella-like Acinetobacter and Streptomyces. Islates grew in a mineral salts medium containing 0.2 mg/ml thiram and no growth factors were required.