INVESTIGATION OF REELIN RS7341475, RS362691 AND RS12705169 GENE POLYMORPHISMS IN CASES A WITH SUICIDE ATTEMPT

Abstract

Background: Suicidal ideations and behavior is one of the most serious conditions among psychiatric emergencies. Neurobiology and genetics of suicide attracted more interest in subsequent period. Today, studies focus on identification of related genes and polymorphisms at chromosomal level as a result of advances in molecular genetics. In this study, we aimed to investigate possible effects of reelin gene polymorphism on the pathogenesis of suicide attempts. Methods: This study included 106 patients with a suicide attempt and 90 healthy controls. Cases with a suicide attempt were assessed and classified by a clinician regarding the reason for a suicide, whether a suicide attempt was planned. The patients attempted to suicide by firearm or ingestion of toxic drug doses were considered as serious suicidal attempt. Reelin rs326691 (G/C), rs734147 (A/G) and rs12705169 (T/G) gene polymorphisms were examined by Real-PCR between patient and control groups. Homozygous mutant, heterozygous and homozygous normal genotypes were identified based on allele discrimination. Results: Of 106 patients with a suicide attempt, 23.6% were men and 76.4% were women. Median age was 20 years (12-52). In our study, it was demonstrated there was a significant difference in genotype and allele distribution of rs734147 gene polymorphism between the patient and control groups (p=0.004 and p=0.003). In addition, it was shown that both rs734147 and rs12705169 gene mutations were significantly more common in cases with serious suicidal attempts intending death when compared to those with suicide attempts seeking attention (p<0.001 and p=0.025). Conclusions: Our results indicated genetic variation of rs734147 and rs12705169 in reelin is significantly associated with the development of suicide attempt.