Lc-ms/ms profiling of 37 fingerprint phytochemicals in oenanthe fistu-losa l. And its biological activities

Abstract

Oenanthe fistulosa L. (Apiaceae) is often associated with damp soils. Its underground parts and the young leaves are mainly cooked with other vegetables. Objective: The aim of the current work was to investigate the chemical profile of dichloromethane (DCM), ethyl acetate (EA) and n-butanol (BuOH) fractions of O. fistulosa through analysis of 37 phytochemicals by LC-MS/MS and to evaluate their biological activities such as antioxidant, anti-cholinesterase and antityrosinase for the first time. Methods: Analysis of 37 phytochemicals was performed by Liquid Chromatography-Mass Spec-trometry (LC-MS/MS). Antioxidant activity was evaluated using five in vitro assays, while anti-cholinesterase and anti-tyrosinase activities were performed using Ellman and Dopachrome meth-ods, respectively. Results: The number of phenolic compounds detected in DCM, EA and BuOH fractions was found to be 9, 15, and 12; respectively. More specifically, 9 phenolic acids were detected and among them, chlorogenic, tr-ferulic and p-coumaric acids were the most abundant. While 8 flavonoids were detected and apigetrin, rutin, and quercitrin were the most abundant. In addition, 3 non-phenolic organ-ic acids (quinic, malic and fumaric acids) were detected in large quantities. Furthermore, the tested plant fractions demonstrated a noteworthy and strong antioxidant action. The plant displayed very strong action against Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes; and BuOH fraction was the most potent one. Finally, BuOH and DCM fractions showed good tyrosinase inhibitory activity. Conclusion: According to the obtained results, O. fistulosa might be a promising candidate for the alleviation of oxidative stress, neurodegenerative (such as Alzheimer’s disease) and hyperpigmenta-tion disorders.